Active Recombinant Mouse Fgf21 Protein (Ala29-Ser210), N-His tagged, Animal-free, Carrier-free

• Cat.No.: Fgf21-257M
• Product Overview: Recombinant Mouse Fgf21 Protein (Ala29-Ser210) with N-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Fibroblast Growth Factors 21 (FGF-21) is a 23 kDa protein with 210 amino acid residues. Belongs to FGF superfamily, FGF-21 is related to glucose and lipid metabolism. Peroxisome proliferator-activated receptor gamma (PPARg) and peroxisome proliferator-activated receptor alpha (PPARa) regulate FGF-21 expression.
• Source: E. coli
• Species: Mouse
• Tag: N-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce proliferation in NIH‑3T3 mouse embryonic fibroblast cells in the presence of mouse Klotho beta and heparin. The ED50 for this effect is < 2 μg/mL.
• Molecular Mass: The protein has a calculated MW of 20.76 kDa.
  The protein migrates as 25-35 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Ala29-Ser210
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Fgf21 fibroblast growth factor 21 [ Mus musculus (house mouse) ]
• Official Symbol: Fgf21
• Synonyms: FGF21; fibroblast growth factor 21; FGF-21
• Gene ID: 56636
• mRNA Refseq: NM_020013
• Protein Refseq: NP_064397
• UniProt ID: Q9JJN1