Active Recombinant Mouse Fgfr3 Protein, His/Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Fgfr3-836MAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant extracellular domain (Met 1-Tyr 367) of mouse FGFR3 (NP_032036.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His/Fc
• Form: Lyophilized
• Bio-activity: Measured by its ability to bind mouse aFGF in a functional ELISA.
• Molecular Mass: The recombinant mouse FGFR3/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 595 amino acids and has a predicted molecular mass of 66 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FGFR3/Fc monomer is approximately 100-110 kDa due to glycosylation.
• Protein length: 595
• N-terminal Sequence Analysis: Glu 21
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 92 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Fgfr3 fibroblast growth factor receptor 3 [ Mus musculus ]
• Official Symbol: Fgfr3
• Synonyms: FGFR3; fibroblast growth factor receptor 3; heparin-binding growth factor receptor; FR3; Mfr3; sam3; CD333; Flg-2; HBGFR; Fgfr-3
• Gene ID: 14184
• mRNA Refseq: NM_001163215
• Protein Refseq: NP_001156687