| Description : |
Interferon-γ is a effective multifunctional cytokine which is released mainly by activated NK cells and T cells. IFN-γ is primarily characterized based on its anti-viral activities, and then been proved to have several functions such as anti-proliferative, immune-regulatory, and pro-inflammatory activities. IFN-γ can upregulate expression of MHC class I and II antigen by antigen-presenting cells. |
| Source : |
Escherichia coli |
| Species : |
Mouse |
| Tag : |
His |
| Form : |
Lyophilized powder |
| AA Sequence : |
MHGTVIESLESLNNYFNSSGIDVEE KSLFLDIWRNWQKDGDMKILQSQII SFYLRLFEVLKDNQAISNNISVIES HLITTFFSNSKAKKDAFMSIA
KFEVNNPQVQRQAFNELIRVVHQLL PESSLRKRKRSRC with polyhistidine tag at the C-terminus |
| Endotoxin : |
<0.01 EU per 1 μg of the protein by the LAL method. |
| Bio-activity : |
Measure by its ability to anti-viral assay in L-929 cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is <0.5 ng/mL. The specific activity of recombinant mouse IFN gamma is approximately >2x 10^6 IU/mg. |
| Purity : |
>98% as determined by SDS-PAGE. Ni-NTA chromatography |
| Storage Buffer : |
The protein was lyophilized from a solution containing 1X PBS, pH 7.4. |
| Reconstitution : |
It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved. |
| Storage : |
Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C. |
| Notes : |
Please use within one month after protein reconstitution. |
