Active Recombinant Mouse Igf2 Protein (Ala25-Glu91), N-His tagged, Animal-free, Carrier-free

• Cat.No.: Igf2-44M
• Product Overview: Recombinant Mouse Igf2 Protein (Ala25-Glu91) with N-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Mouse Insulin Like Growth Factors 2 (IGF-II) is a 7.34 kDa member of the Insulin-like Growth Factors with 67 amino acid residues. IGF-II is mainly expressed from early embryonic somatic tissues, liver, and epithelial cells lining the surface of the brain. IGF-II is mediating the embryonic development and placental growth, while also promoting tumor growth. IGF-II affects regulation of cell proliferation, growth, migration, differentiation and survival via binding to IGF-I receptor.
• Source: E. coli
• Species: Mouse
• Tag: N-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce MCF-7 cells proliferation. The ED50 for this effect is < 6 ng/mL. The specific activity of recombinant mouse IGF-II is > 1.5 × 10⁵ IU/mg.
• Molecular Mass: The protein has a calculated MW of 8.20 kDa.
  The protein migrates about 11 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Ala25-Glu91
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 2 weeks under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Igf2 insulin-like growth factor 2 [ Mus musculus (house mouse) ]
• Official Symbol: Igf2
• Synonyms: IGF2; insulin-like growth factor 2; insulin-like growth factor II; multiplication-stimulating polypeptide; Mpr; M6pr; Peg2; Igf-2; Igf-II; AL033362
• Gene ID: 16002
• mRNA Refseq: NM_001122736
• Protein Refseq: NP_001116208
• UniProt ID: P09535