Description : |
Leukemia Inhibitory Factor (LIF) is a pleiotropic cytokine belonging to the long four-helix bundle cytokine superfamily. LIF shares tertiary structure with several other cytokines, including Interleukin-6 (IL-6), Oncostatin M, ciliary neurotropic factor, and cardiotrophin-1, and their functions in vivo are also redundant to some extent. LIF can bind to the common receptor of IL-6 subfamily, gp130, and then recruit its own receptor LIF Receptor to form a ternary complex. The basal expression of LIF in vivo is low; and its expression is induced by pro-inflammatory factors, including lipopolysaccharide, IL-1, and IL-17, and inhibited by anti-inflammatory agents, including IL-4 and IL-13. The functions of LIF include proliferation of primordial germ cells, regulation in blastocyst implantation and early pregnancy, and maintenance of pluripotent embryonic stem cells. |
Source : |
E. coli |
Species : |
Mouse |
Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Bio-activity : |
ED50 < 0.01 ng/mL, measured by a cell differentiation assay using M1 cells, corresponding to a specific activity of > 1.0 × 10^8 units/mg. |
Molecular Mass : |
19.9 kDa, observed by reducing SDS-PAGE. |
Protein Length : |
180 |
AA Sequence : |
SPLPITPVNATCAIRHPCHGNLMNQ IKNQLAQLNGSANALFISYYTAQGE PFPNNVEKLCAPNMTDFPSFHGNGT EKTKLVELYRMVAYLSASLTNITRD QKVLNPTAVSLQVKLNATIDVMRGL LSNVLCRLCNKYRVGHVDVPPVPDH SDKEAFQRKKLGCQLLGTYKQVISV VVQAFSPLPITPVNATCAIRHPCHG NLMNQ IKNQLAQLNGSANALFISYYTAQGE PFPNNVEKLCAPNMTDFPSFHGNGT EKTKLVELYRMVAYLSASLTNITRD QKVLNPTAVSLQVKLNATIDVMRGL LSNVLCRLCNKYRVGHVDVPPVPDH SDKEAFQRKKLGCQLLGTYKQVISV VVQAF |
Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
Purity : |
> 95% by SDS-PAGE and HPLC analyses. |
Storage : |
Lyophilized recombinant mouse Leukemia Inhibitory Factor (rmLIF) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rmLIF should be stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade. |
Storage Buffer : |
Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH8.0. |
Reconstitution : |
Reconstituted in ddH2O at 100 μg/mL. |