Active Recombinant Mouse Lif Protein (Ser24-Phe203), N-His tagged, Animal-free, Carrier-free

• Cat.No.: Lif-65M
• Product Overview: Recombinant Mouse Lif Protein (Ser24-Phe203) with N-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein, belonging to the IL-6 receptor family. Mouse LIF shares 79% sequence homology with human LIF. LIF is a 19.7 kDa protein containing 202 amino acid which high expression in human liver, bone, uterus, kidney and the central nervous system. LIF is a inducer of differentiation in M1 leukaemia cells, osteoblasts and glia. Not only stimulates proliferation of DA1 cells inhibit proliferation of corticotrophs and promote cell survival in some cell types but also induces apoptosis in others.
• Source: E. coli
• Species: Mouse
• Tag: N-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce IL-6 secretion in M1 cells. The ED50 for this effect is < 0.5 ng/mL. The specific activity of recombinant mouse LIF is > 2 × 10⁶ IU/mg.
• Molecular Mass: The protein has a calculated MW of 20.68 kDa.
  The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Ser24-Phe203
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >95% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Lif leukemia inhibitory factor [ Mus musculus (house mouse) ]
• Official Symbol: Lif
• Synonyms: LIF; leukemia inhibitory factor; d factor; differentiation-stimulating factor
• Gene ID: 16878
• mRNA Refseq: NM_001039537
• Protein Refseq: NP_001034626
• UniProt ID: P09056