Active Recombinant Mouse MET protein, Fc-tagged

• Cat.No.: MET-1558M
• Product Overview: Recombinant Mouse MET protein(Glu25-Ala931), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5.
• Bio-activity: Immobilized Human HGF, His Tag at 2 μg/mL (100 μL/well) can bind Mouse HGF R, Fc Tag with a linear range of 0.2-10 ng/mL.
• Molecular Mass: Mouse HGF R, Fc Tag is fused with Fc fragment of human IgG1 at the C-terminus. The mature form of HGF R is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 32.1 kDa (α chain) and 95.1 kDa (β chain Fc chimera). The protein migrates as 40-45 kDa (α chain) and 125-135 kDa (β chain Fc chimera) under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Glu25-Ala931
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Met met proto-oncogene [ Mus musculus ]
• Official Symbol: MET
• Synonyms: MET; met proto-oncogene; hepatocyte growth factor receptor; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase; HGF; HGFR; Par4; c-Met; AI838057
• Gene ID: 17295
• mRNA Refseq: NM_008591
• Protein Refseq: NP_032617