Active Recombinant Mouse Msln protein, His-tagged
Cat.No. : | Msln-32M |
Product Overview : | Recombinant Mouse Msln(Asp298-Ser600) fused with His tag at C-terminal was expressed in CHO. |
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Description : | Mesothelin, also known as CAK1 and ERC, is derived from a 70 kDa precursor that also includes Megakaryocyte Potentiating Factor (MPF). The 70 kDa precursor is expressed on the cell surface where it is cleaved at a dibasic proteolytic site to release the 32 kDa glycosylated MPF. MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation. The term Mesothelin refers to the 40 kDa glycosylated protein which remains attached to the cell surface via a GPI linkage. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas. It is up-regulated in mesotheliomas and a range of carcinomas and adenomas. Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression, in part through the up-regulation of MMP-7. It is co-expressed with the tumor antigen CA125/MUC16 on advanced ovarian adenocarcinomas and interacts with this molecule to support cell adhesion. A soluble form of Mesothelin, with its GPI anchor intact, is released from tumor cells and binds to MMR/CD206 on macrophages. |
Source : | CHO |
Species : | Mouse |
Tag : | His |
Predicted N Terminal : | Asp298 |
Form : | Lyophilized from a 0.2 μm filtered solution in PBS with trehalose. |
Bio-activity : | Measured by its binding ability in a functional ELISA. When Recombinant Mouse Mesothelin is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human CA125/MUC16 that produces 50% optimal binding response is approximately 0.4-2.4 ng/mL. |
Molecular Mass : | Predicted Molecular Mass: 35 kDa; SDS-PAGE: 38-55 kDa, reducing conditions. |
Protein length : | Asp298-Ser600 |
Endotoxin : | <0.10 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE with silver staining |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | Reconstitute at 250 μg/mL in PBS. |
Shipping : | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Gene Name : | Msln mesothelin [ Mus musculus ] |
Official Symbol : | Msln |
Synonyms : | MSLN; mesothelin; megakaryocyte potentiating factor; pre-pro-megakaryocyte-potentiating factor; MPF; |
Gene ID : | 56047 |
mRNA Refseq : | NM_018857 |
Protein Refseq : | NP_061345 |
UniProt ID : | Q61468 |
Chromosome Location : | 17; 17 A3.3 |
Products Types
◆ Lysates | ||
MSLN-1343HCL | Recombinant Human MSLN cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionMSLN is normally expressed in mesothelial cells, playing a role in cell adhesion.
In cancer, MSLN expression is significantly upregulated.
MSLN is overexpressed in mesothelioma, ovarian, and pancreatic cancers.
Therapies targeting MSLN include antibody-drug conjugates and CAR-T cell therapies.
MSLN levels can be assessed through immunohistochemistry and serum biomarker tests.
MSLN overexpression is often associated with poor prognosis in cancer patients.
MSLN promotes tumor cell proliferation, invasion, and resistance to apoptosis.
Customer Reviews (3)
Write a reviewVery satisfied, great product.
Product is high-quality.
Excellent for experiments, reliable.
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