Active Recombinant Mouse NOTCH1 protein, His-tagged

• Cat.No.: NOTCH1-0570M
• Product Overview: Recombinant Mouse NOTCH1 protein(Ala19-Gln526), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human DLL4, Fc Tag at 5 μg/mL (100 μL/well) can bind Mouse NOTCH1, His Tag with a linear range of 0.02-0.313 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 55.4 kDa. The protein migrates as 58-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ala19-Gln526
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Notch1 Notch gene homolog 1 (Drosophila) [ Mus musculus ]
• Official Symbol: NOTCH1
• Synonyms: NOTCH1; Notch gene homolog 1 (Drosophila); neurogenic locus notch homolog protein 1; mT14; p300; Motch A; notch 1; major type A protein; transmembrane receptor Notch1; N1; Mis6; Tan1; lin-12; 9930111A19Rik
• Gene ID: 18128
• mRNA Refseq: NM_008714
• Protein Refseq: NP_032740