Active Recombinant Mouse Pvrl2 protein(Met1-Gly351), His-tagged

• Cat.No.: Pvrl2-2269M
• Product Overview: Recombinant Mouse PVRL2 (NP_033016.3)(Met 1-Gly 351) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Gly351
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. 1. Immobilized mouse CD112-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse DNAM1-His with a linear range of 0.156-5.0 μg/ml. 2. Immobilized mouse CD112-His at 10 μg/ml (100 μl/well) can bind mouse DNAM1-Fc with a linear range of 0.03-1.0 μg/ml.
• Molecular Mass: The secreted recombinant mouse PVRL2 comprises 331 amino acids with a predicted molecular mass of 36 kDa. As a result of glycosylation, it migrates as an approximately 40-45 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Pvrl2 poliovirus receptor-related 2 [ Mus musculus ]
• Official Symbol: Pvrl2
• Synonyms: PVRL2; poliovirus receptor-related 2; poliovirus receptor-related protein 2; hveB; mHveB; poliovirus sensitivity; poliovirus receptor homolog; herpesvirus entry mediator B; herpes virus entry mediator B; murine herpesvirus entry protein B; murine herpes virus entry protein B; MPH; Pvr; Pvs; Cd112; AI325026; AI987993; nectin-2
• Gene ID: 19294
• mRNA Refseq: NM_001159724
• Protein Refseq: NP_001153196