Active Recombinant Mouse TNF-β Protein, His-Tagged

• Cat.No.: TNF-β-01M
• Product Overview: Recombinant mouse TNF-β Protein, His-tagged was expressed in E. coli cell

Specification

• Description: TNF-β works as a potential mediator in the inflammatory and immune process. It a component of the TNF family of ligands, and signals through TNFR1 and TNFR2. TNF-β is secreted by activated T and B lymphocytes, and has similar function to TNF-α. In the same manner as TNF-α, TNF-β is involved in the regulation of various biological processes, including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNF-β is generally released as a soluble polypeptide. In addition, lymphotoxin-β can anchor TNF-β to the cell surface and form heterotrimers in an effective manner. TNF-β is cytotoxic to a wide range of tumor cells.
• Source: Escherichia coli
• Species: Mouse
• Tag: His
• Form: Lyophilized powder
• AA Sequence: LSGVRFSAARTAHPLPQKHLTHGIL KPAAHLVGYPSKQNSLLWRASTDRA FLRHGFSLSNNSLLIPTSGLYFVYS QVVFSGESCSPRAIPTPIYL
  AHEVQLFSSQYPFHVPLLSAQKSVY PGLQGPWVRSMYQGAVFLLSKGDQL STHTDGISHLHFSPSSVFFGAFAL with polyhistidine tag
  at the N-terminus
• Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
• Bio-activity: Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is <30 ng/mL
• Purity: >98% as determined by SDS-PAGE. Ni-NTA chromatography
• Storage Buffer: The protein was lyophilized from a solution containing 1X PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Storage: Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
• Notes: Please use within one month after protein reconstitution.

Gene Information

• Gene Name: Lta lymphotoxin A [ Mus musculus (house mouse) ]
• Official Symbol: TNF-β
• Synonyms: LT; Ltx; Tnfb; LT[a]; LT-[a]; TNFSF1; Tnlg1e; hlb382; LTalpha; Tnfsf1b; LT-alpha; TNF-beta
• Gene ID: 16992
• mRNA Refseq: NM_010735.2
• Protein Refseq: NP_034865.1
• UniProt ID: P09225