Active Recombinant Pig CYP2E1 Protein

• Cat.No.: CYP2E1-134P
• Product Overview: Recombinant pig CYP2E1 protein and CYP-reductase without tag were coexpressed in E. coli.

Specification

• Description: A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics.
• Source: E. coli
• Species: Pig
• Bio-activity: Cytochrome c Reductase Activity: 367 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Chlorzoxazone 6-hydroxylase
  Linear with time up to: 15 min(Determined at 100 µM substrate, using a P450 concentration of 100 pmol/ml)
  Linear with P450 up to: 160 pmol/ml(Determined at 100 µM substrate, using an incubation time of 5 min)
  Vmax: 3.2 pmol/min/pmol CYP
  Km: 132 µM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 9.9 nmol/ml
  Cytochrome b5 Concentration: 49.4 nmol/ml
  Protein Concentration: 17.7 mg/ml
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP2E1 cytochrome P450, family 2, subfamily E, polypeptide 1 [ Sus scrofa (pig) ]
• Official Symbol: CYP2E1
• Synonyms: CYP2E1; cytochrome P450, family 2, subfamily E, polypeptide 1; cytochrome P450 2E1; 4-nitrophenol 2-hydroxylase; CYPIIE1; cytochrome P-450-J; cytochrome P450-J
• Gene ID: 403216
• mRNA Refseq: NM_214421
• Protein Refseq: NP_999586
• UniProt ID: P79383