| Description : |
Ciliary Neurotrophic Factor (CNTF) is a polypeptide hormone which acts within the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. CNTF is a potent survival factor for neurons and oligodendrocytes and may play a role in reducing tissue damage during increased inflammation. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, however this phenotype is not causally related to neurologic disease. |
| Source : |
E. coli |
| Species : |
Rat |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50 < 30 ng/mL, measured by its ability to induce alkaline phosphatase production byTF-1 Cells. |
| Molecular Mass : |
22.9 kDa, observed by reducing SDS-PAGE |
| Protein Length : |
199 |
| AA Sequence : |
AFAEQTPLTLHRRDLCSRSIWLARK IRSDLTALMESYVKHQGLNKNINLD SVDGVPVASTDRWSEMTEAERLQEN LQAYRTFQGMLTKLLEDQRVHFTPT EGDFHQAIHTLMLQVSAFAYQLEEL MVLLEQKIPENEADGMPATVGDGGL FEKKLWGLKVLQELSQWTVRSIHDL RVISSHQMGISALESHYGAKDKQM |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 95% as analyzed by SDS-PAGE. |
| Storage : |
Lyophilized recombinant Rat CNTF remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rrCNTF should be stable up to 1 week at 4 centigrade or up to 3 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against 50mM Tris, pH 8.0. |
| Reconstitution : |
Reconstituted in ddH2O at 100 μg/mL. |
