| Description : |
Epidermal Growth Factor, a low-molecular-weight polypeptide, is the founding member of the EGF-family of proteins. It can be found in platelets, macrophages, urine, saliva, etc. EGF acts by binding with high affinity to the Epidermal Growth Factor Receptor (EGFR) and stimulating downstream protein tyrosine kinase activity. This signal transduction cascade results in increased intracellular calcium levels and increased rates of glycolysis and protein synthesis. EGF stimulates the growth of many epidermal and epithelial tissues. Pharmaceutical drugs designed to inhibit EGFR have been used to treat certain types of cancer. |
| Source : |
CHO |
| Species : |
Rat |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50 < 0.1 ng/mL, measured in a cell proliferation assay using 3T3 cells. |
| Molecular Mass : |
~6 kDa, observed by reducing SDS-PAGE. |
| AA Sequence : |
MNSNTGCPPSYDGYCLNGGVCMYVE SVDRYVCNCVIGYIGERCQHRDLRW WKLR |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 95% as analyzed by SDS-PAGE and HPLC. |
| Storage : |
Lyophilized recombinant Rat Epidermal Growth Factor remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, Rat Epidermal Growth Factor should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O or PBS at 100 μg/mL. |
