Active Recombinant Rat FAP protein, His-tagged

• Cat.No.: FAP-1442R
• Product Overview: Recombinant Rat FAP protein(Leu26-Asp761), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Rat
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
• Bio-activity: Immobilized Rat FAP, His Tag at 1 μg/mL (100 μL/well) can bind Anti-FAP Antibody, Mouse IgG2a (FAP5) with a linear range of 0.6-4 ng/mL.Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >6500 pmol/min/μg.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminus.The protein has a calculated MW of 91.5 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Leu26-Asp761
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Fap fibroblast activation protein, alpha [ Rattus norvegicus ]
• Official Symbol: FAP
• Synonyms: FAP; fibroblast activation protein, alpha; seprase
• Gene ID: 192203
• mRNA Refseq: NM_138850
• Protein Refseq: NP_620205

Reviews

08/11/2020

It enables accurate detection and quantification of FAP protein levels in samples, providing crucial insights into its expression, regulation, and potential functional changes under different experimental conditions.

05/04/2020

FAP protein is highly recommended for researchers due to its excellent performance in various assays, particularly in Western blotting (WB) experiments, and its applicability in protein electron microscopy structure analysis.

01/02/2019

FAP protein is highly recommended for researchers, as it demonstrates excellent performance in WB experiments and has proven useful in protein electron microscopy structure analysis.

Q&As

How does FAP contribute to immunosuppression in the tumor microenvironment?

FAP can suppress the immune response by modulating the activity of certain immune cells, creating an immunosuppressive tumor microenvironment.

How can FAP expression be used as a prognostic marker in cancer patients?

High levels of FAP expression in tumors have been associated with poor prognosis, making it a potential prognostic marker for certain cancers.

Are there ongoing clinical trials exploring FAP-targeted therapies?

Yes, several clinical trials are investigating the safety and efficacy of FAP-targeted therapies in various cancer types.

Can FAP be utilized as a therapeutic target beyond cancer treatment?

FAP-targeted therapies are being explored for conditions such as fibrotic diseases and rheumatoid arthritis, extending its potential clinical applications.

How is imaging technology utilized to detect FAP expression in tumors?

Molecular imaging techniques, such as positron emission tomography (PET), can be employed to visualize FAP expression in tumors noninvasively.