Active Recombinant Rat ICOS protein, His-tagged

• Cat.No.: ICOS-0230R
• Product Overview: Recombinant Rat ICOS protein(Glu21-Pro145), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Rat
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Rat ICOS / CD278 (C137S, C138S), His Tag at 5 μg/mL (100 μL/well) can bind Mouse B7-H2, Fc Tag with a linear range of 2-39 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 15.9 kDa. The protein migrates as 24-26 kDa and 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Glu21-Pro145
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Icos inducible T-cell co-stimulator [ Rattus norvegicus ]
• Official Symbol: ICOS
• Synonyms: ICOS; inducible T-cell co-stimulator; inducible T-cell costimulator; activation-inducible lymphocyte immunomediatory molecule; Ailim
• Gene ID: 64545
• mRNA Refseq: NM_022610
• Protein Refseq: NP_072132