Source : |
HEK293 |
Species : |
Rat |
Tag : |
Fc |
Form : |
Lyophilized from 0.22 μm filtered solution in 51 mM Tris,100 mM Glycine,25 mM Arginine,150 mM NaCl,pH7.5 with trehalose as protectant. |
Bio-activity : |
Immobilized Mouse ICOS (C137S, C138S), His Tag at 5 μg/mL (100 μL/well) can bind Rat B7-H2, Fc Tag with a linear range of 0.6-39 ng/mL.Immobilized Biotinylated Human ICOS (C136S, C137S), His,Avitag (recommended for biopanning) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Rat B7-H2, Fc Tag with a linear range of 2-39 ng/mL. |
Molecular Mass : |
This protein carries a human IgG1 Fc tag at the C-terminusThe protein has a calculated MW of 53.4 kDa. The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein Length : |
Glu 25-Lys 261 |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>90% as determined by SDS-PAGE. |
Storage : |
For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |