Active Recombinant Rat ROCK1(2-543) Protein, N-His,N-HA-tagged

• Cat.No.: ROCK1-216H
• Product Overview: N-terminal 6His-tagged and N-terminal HA tagged, recombinant, rat ROKα/ROCKII, amino acids 2–543. Expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: Rho-associated, coiled-coil containing protein kinase (ROCK), including the ROCK1 and ROCK2 isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. PKA and the two ROCKs have been reported to have opposite effects on neurite formation. More studies are needed to provide significant insights into the role of ROCK in several pathological processes. ROCK inhibitors have been shown the benefit for treatment of various cancers, inflammation, coronary artery spasms and hypertension.
• Source: Sf21
• Species: Human
• Tag: His,HA
• Protein length: 2-543 aa
• Form: 4.35mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 2mM benzamidine, 0.4mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 1649U/mg, where one unit of ROKα/ROCKII activity is defined as 1nmol phosphate incorporated into 30μM long S6 substrate peptide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 67.2 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMGSEF ATMYPYDVPD YAPGAPEAAP GDGAGAGRQR 61 KLEALIRDPR SPINVESLLD GLNSLVLDLD FPALRKNKNI DNFLNRYEKI VKKIRGLQMK 121 AEDYDVVKVI GRGAFGEVQL VRHKASQKVY AMKLLSKFEM IKRSDSAFFW EERDIMAFAN 181 SPWVVQLFCA FQDDRYLYMV MEYMPGGDLV NLMSNYDVPE KWAKFYTAEV VLALDAIHSM 241 GLIHRDVKPD NMLLDKHGHL KLADFGTCMK MDETGMVHCD TAVGTPDYIS PEVLKSQGGD 301 GYYGRECDWW SVGVFLFEML VGDTPFYADS LVGTYSKIMD HKNSLCFPED TEISKHAKNL 361 ICAFLTDREV RLGRNGVEEI KQHPFFKNDQ WNWDNIRETA APVVPELSSD IDSSNFDDIE 421 DDKGDVETFP IPKAFVGNQL PFIGFTYFRE NLLLSDSPPC RENDAIQTRK SEESQEIQKK 481 LYALEEHLSS EVQAKEELEQ KCKSINTRLE KTAKELEEEI TFRKNVESTL RQLEREKALL 541 QHKNAEYQRK ADHEADKKRN LENDVNSLKD QLEDLKKRNQ SSQI
• Purity: Purity 63% by SDSPAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: Rock1 Rho-associated coiled-coil containing protein kinase 1 [ Mus musculus (house mouse) ]
• Official Symbol: ROCK1
• Synonyms: ROCK1; Rho associated coiled-coil containing protein kinase 1; Rock-I; 1110055K06Rik
• Gene ID: 19877
• mRNA Refseq: NM_009071.2
• Protein Refseq: NP_033097.1
• UniProt ID: P70335

Reviews

08/20/2022

Reliable protein crystallization services, consistently delivers exceptional results.

03/06/2022

Accurate protein structural characterization, vital for our investigations.

07/14/2021

High-quality protein sequencing, an essential tool in our lab.

Q&As

What role does ROCK1 play in vascular tone regulation and cardiovascular health?

ROCK1 plays a significant role in vascular tone regulation, impacting blood pressure and cardiovascular function.

What are the mechanisms by which ROCK1 modulates inflammatory responses in the body?

ROCK1 regulates inflammatory processes, influencing immune cell activity and inflammation.

What is the impact of ROCK1 on cancer cell invasion, metastasis, and tumor progression?

ROCK1 influences cancer progression, particularly in aspects of cell invasion, metastasis, and tumor growth.

How does ROCK1 (Rho-Associated Coiled-Coil Containing Protein Kinase 1) regulate cytoskeletal dynamics and cell motility?

ROCK1 controls cytoskeletal rearrangements, crucial for cell shape, movement, and division.

How does ROCK1 contribute to the pathogenesis of neurological disorders, such as Alzheimer's disease?

ROCK1 is involved in neurological disorders by affecting neuronal cytoskeletal dynamics and synaptic function.

How does ROCK1 influence the process of apoptosis and cell survival?

ROCK1 modulates apoptosis, playing a role in determining cell survival or programmed cell death.

How do alterations in ROCK1 expression or activity affect tissue remodeling and fibrosis?

Changes in ROCK1 activity impact tissue remodeling and fibrosis, affecting wound healing and disease progression.