Active Recombinant Rat Rps6kb1(T412E), His-tagged

• Cat.No.: Rps6kb1-143R
• Product Overview: Recombinant Rat Rps6kb1 (amino acid residues 1-421), with N-terminal 6His, was expressed in insect cells. p70 S6 kinase has a T412E mutation to mimic the activation of the enzyme through phosphorylation of Thr412 by PDK2.

Specification

• Description: Protein ubiquitylation and protein phosphorylation are the two major mechanisms that regulate the functions of proteins in eukaryotic cells.
• Source: Insect cells
• Species: Rat
• Tag: His
• Form: 50 mM Tris/HCl pH7.5, 0.1mM EGTA, 150 mM NaCl, 0.1% ?-Mercaptoethanol, 270 mM sucrose, 0.03% Brij-35, 1 mM Benzamidine, 0.2 mM PMSF.
• Bio-activity: 154.2 Units/mg (169.6 Units/ml)Substrate: CROSStide (GRPRTSSFAEG)
• Molecular Mass: ~48.6 kDa
• Purity: >95% by InstantBlue™ SDS-PAGE
• Unit Definition: 1 Unit = 1 nmole of phosphate incorporated into the substrate in 1 minute.
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 1.1 mg/ml

Gene Information

• Gene Name: Rps6kb1 ribosomal protein S6 kinase, polypeptide 1 [ Rattus norvegicus ]
• Official Symbol: Rps6kb1
• Synonyms: RPS6KB1; ribosomal protein S6 kinase, polypeptide 1; ribosomal protein S6 kinase beta-1; S6K; S6K1; P70S6K; P70S6K1; p70 S6KA; S6 kinase; p70-S6K 1; S6K-beta-1; p70 S6K-alpha; p70(S6K)-alpha; p70 S6 kinase alpha; p70 ribosomal S6 kinase alpha; ribosomal protein S6 kinase I; 70 kDa ribosomal protein S6 kinase 1; ribosomal protein S6 kinase, 70kDa, polypeptide 1
• Gene ID: 83840
• mRNA Refseq: NM_031985
• Protein Refseq: NP_114191
• Pathway: Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; Cytoplasmic Ribosomal Proteins, organism-specific biosystem; ErbB signaling pathway, organism-specific biosystem; ErbB signaling pathway, organism-specific biosystem; ErbB signaling pathway, conserved biosystem
• Function: ATP binding; ATP binding; nucleotide binding; peptide binding; protein binding; protein kinase activity; protein kinase activity; protein kinase activity; ribosomal protein S6 kinase activity; transferase activity, transferring phosphorus-containing groups

Reviews

09/16/2021

Dependable protein purification, consistently high-quality results.

06/11/2021

Precise mass spectrometry analysis, essential for our proteomics work.

06/30/2019

Efficient peptide synthesis, crucial for our peptide research projects.

Q&As

What are the mechanisms by which RPS6KB1 modulates autophagy and cell survival?

RPS6KB1 regulates autophagy and cell survival, crucial for cellular health and response to stress.

How does RPS6KB1 (Ribosomal Protein S6 Kinase B1) regulate protein synthesis and cell growth?

RPS6KB1 controls protein synthesis and cell growth, playing a key role in cellular proliferation.

What is the impact of RPS6KB1 on cellular responses to stress and DNA damage?

RPS6KB1 impacts cellular stress responses, including the repair of DNA damage and maintenance of genomic stability.

How does RPS6KB1 contribute to the development and progression of cancer?

RPS6KB1 can contribute to cancer by promoting tumor cell growth and proliferation.

What role does RPS6KB1 play in the mTOR signaling pathway, particularly in response to nutrient availability?

RPS6KB1 is a critical component of the mTOR pathway, responding to nutrient signals to regulate cell metabolism and growth.

How do alterations in RPS6KB1 expression or activity affect aging and age-related diseases?

Changes in RPS6KB1 function can affect aging processes and the development of age-related diseases.

How does RPS6KB1 influence metabolic processes, especially in conditions like obesity and diabetes?

RPS6KB1 influences metabolic processes, affecting how cells respond to conditions like obesity and diabetes.