Active Recombinant Rat TIGIT protein(Met1-Ala138), hFc-tagged

• Cat.No.: TIGIT-0802R
• Product Overview: Recombinant Rat TIGIT (XP_008766987.1) (Met1-Ala138) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: C-hFc
• Protein length: Met1-Ala138
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Nectin-2 Protein, Human, Recombinant (ECD, His Tag) at 2 μg/ml (100 μl/well) can bind TIGIT Protein, Rat, Recombinant (hFc Tag), the EC50 of TIGIT Protein, Rat, Recombinant (hFc Tag)is 60-300 ng/mL.
• Molecular Mass: The recombinant Rat TIGIT consists of 360 amino acids and predicts a molecular mass of 40.02 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Tigit T cell immunoreceptor with Ig and ITIM domains [ Rattus norvegicus ]
• Official Symbol: TIGIT
• Synonyms: TIGIT; T cell immunoreceptor with Ig and ITIM domains; RGD1563191
• Gene ID: 363784