Active Recombinant Rhesus macaque CD4 protein, His-tagged

• Cat.No.: CD4-377R
• Product Overview: Recombinant Rhesus macaque CD4 protein(Lys 26 - Trp 390), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Rhesus macaque
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Ibalizumab at 1 μg/mL (100 μL/well) can bind Rhesus macaque CD4, His Tag with a linear range of 0.8-13 ng/mL.Immobilized Rhesus macaque CD4, His Tag at 1 μg/mL (100 μL/well) can bind Ibalizumab with a linear range of 0.2-13 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 42.3 kDa. The protein migrates as 50-57 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Lys26-Trp390
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Reviews

01/08/2031

The CD4 proteins purchased from your company is suitable for ELISA.

02/06/2023

Protein products are put into experiments with good effect and reliable experimental results.

01/22/2022

The technical expert will follow up on the progress of my experiments and provide constructive advice on the problems that arise in my experiments.

Q&As

What is the connection between CD4 and glycoprotein GP120?

CD4 binds to glycoprotein GP120.

Where is CD4 primarily expressed?

CD4 is mainly expressed in T cells and thymocytes.

Where is CD4 localized?

CD4 is localized in the cell membrane.