Active Recombinant Rhesus macaque MUC16 protein, His-tagged

• Cat.No.: MUC16-2977R
• Product Overview: Recombinant Rhesus macaque MUC16 protein(Pro 13647-Pro 14291), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Rhesus macaque
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Rhesus macaque CA125 (13647-14291), His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human Mesothelin, Fc,Avitag with a linear range of 0.002-0.313 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 73.9 kDa. The protein migrates as 95-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Pro 13647-Pro 14291
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Reviews

07/28/2020

Critical for decision-making. Supports our research endeavors.

05/24/2018

Expert service quality. Valuable for research integrity.

06/05/2017

Dependable partner in experiments. Consistent and trustworthy.

Q&As

How does MUC16 interact with other components of the epithelial barrier and immune system?

MUC16 works with other mucins and immune components to maintain epithelial barrier function and immune responses.

How does MUC16 contribute to the formation of the mucosal barrier?

It aids in forming the mucosal barrier, protecting against pathogens and mechanical damage.

What potential therapeutic applications arise from targeting MUC16 in cancer treatment?

Targeting MUC16 in cancer therapy, especially in ovarian cancer, could be effective in inhibiting tumor growth and spread.

What is the impact of altered MUC16 activity on inflammatory and infectious diseases?

Altered MUC16 activity can impact the body's defense against inflammation and infection.

What is the primary role of MUC16 in epithelial cell function?

MUC16, a mucin protein, is essential for maintaining the protective mucous layer on epithelial surfaces.

What role does MUC16 play in cancer, particularly ovarian cancer?

MUC16 is associated with cancer progression, particularly in ovarian cancer, where it may facilitate tumor growth and metastasis.

How do genetic variations in MUC16 affect its function in mucosal protection and cancer development?

Genetic variations in MUC16 can lead to differences in mucosal integrity and potentially influence cancer susceptibility.