Active Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD(Y453F) Protein, His-tagged
Cat.No. : | S-475S |
Product Overview : | Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD(Y453F)-His Recombinant Protein (YP_009724390.1) (Arg319-Phe541(Y453F)) was expressed in HEK293 cell with a polyhistidine tag at the C-terminus. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | SARS-CoV-2 |
Tag : | His |
Predicted N Terminal : | Arg 319 |
Bio-activity : | Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(Y453F)-His, the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD(Y453F)-His is 5-20 ng/mL. |
Molecular Mass : | The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD(Y453F)-His Recombinant Protein consists of 234 amino acids and predicts a molecular mass of 26.5 kDa. As a result of glycosylation, it migrates as an approximately 35.6 kDa band in SDS-PAGE under reducing conditions. |
Protein length : | 319-541 |
Endotoxin : | < 1.0 EU/μg protein as determined by the LAL method. |
Purity : | > 95 % as determined by SDS-PAGE. |
Storage : | Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Storage Buffer : | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. |
Gene Name : | S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] |
Official Symbol : | S |
Synonyms : | S; surface glycoprotein; spike glycoprotein; surface glycoprotein; structural protein; spike protein |
Gene ID : | 43740568 |
mRNA Refseq : | MN908947 |
Protein Refseq : | YP_009724390 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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