Active Recombinant Schistosoma japonicum GST, His-tagged
Cat.No. : | GST-112S |
Product Overview : | Recombinant Schistosoma japonicum GST full length protein contains 244 amino acids(1-224 a.a.) expressed in E.coli, having a molecular mass of 28.3kDa. The GST protein is fused to 20 amino acid His-Tag at N-terminus. The GST protein is purified by proprietary chromatographic techniques. |
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Description : | Antioxidant enzyme Glutathione S- Transferase (GST) is thought to do the primary cellular defense mechanism against reactive oxygen species. GST reduces lipid hydroperoxides through its Se-independent glutathione peroxidase activity. The enzyme also detoxifies lipid peroxidation end products such as 4-hydroxynonenal (4-HNE). The soluble GST is a 26 kDa protein which occurs as a dimer in all aerobic organisms. Each monomer has two domains, one that binds GSH and is an /-structure similar to thioredoxin and the other, all helical, that binds the hydrophobic substrate. The GST -fusion protein expression system is a widely used recombinant protein expression system that allows a peptide or a regulatory protein domain to be expressed as a fusion to the C-terminus of Schistosoma japonicum GST. Fusion proteins also possess GST -enzymatic activity and can undergo dimerization similar to in vivo. The fusion protein can be purified via GST -affinity column chromatography. In most cases, the desired peptides or domains are removed from GST by applying a specific protease that recognizes and cleaves the linker between the protein domain and GST. The technique has been widely used to generate different kinds of proteins for crystallization, molecular immunology studies, the production of vaccines and studies involving protein-protein and protein-DNA interactions. |
Source : | E.coli |
Species : | Schistosoma japonicum |
Tag : | His |
Form : | Sterile Filtered clear solution. GST is supplied in PBS pH 7.4 & 10% glycerol. |
Bio-activity : | 2.8-3.3 units/mg, & is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH-6.5 at 25C. |
Molecular Mass : | 28.3 kDa |
AA Sequence : | MGSSHHHHHH SSGLVPRGSH MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMAIIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALDVVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR. |
Purity : | Greater than 95% as determined by SDS-PAGE. |
Stability : | Store vial at -20°C to -80°C. When stored at the recommended temperature, this protein is stable for 12 months. Please prevent freeze-thaw cycles. |
Products Types
◆ Recombinant Protein | ||
GST-2758P | Recombinant Pan-species (General) GST Protein, His-tagged | +Inquiry |
GST-1268S | Recombinant Schistosoma japonicum GST protein(Met1-Lys218) | +Inquiry |
GST-8543S | Recombinant Schistosoma japonicum GST Protein | +Inquiry |
GST-2759P | Recombinant Pan-species (General) GST Protein, N-GST-tagged | +Inquiry |
GST-001S | Active Recombinant S. japonicum GST Protein | +Inquiry |
◆ Lysates | ||
GST-573SCL | Recombinant Schistosoma japonicum GST cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-0397 | Fluoro GST Activity Kit | +Inquiry |
Kit-0399 | GST Activity Colorimetric Assay Kit | +Inquiry |
Kit-0398 | Glutathione S-Transferase Assay Kit | +Inquiry |
Kit-0400 | GST Activity Fluorometric Assay Kit | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (4)
Ask a questionGST is mainly localized in the cytoplasm.
Stress stimuli contributed to the up-regulation of GST gene expression, and GSTs showed higher enzymatic activities under different stress stimuli.
GST plays an important role in phytoremediation, detoxification of xenobiotics, and oxidative stress metabolism.
GST proteins are usually composed of homo- or heterodimers, and each subunit constituting a dimer contains two structural domains, the N-terminal and C-terminal domains.
Customer Reviews (3)
Write a reviewExcellent after-sales service, solving many experimental problems.
The GST protein I purchased is suitable for WB, and I have obtained satisfactory experimental results.
The protein composition was stable and concentrated, allowing me to obtain satisfactory experimental results.
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