Active Recombinant TEV Protease Protein (231 aa), N-His-tagged
Cat.No. : | Protease-308P |
Product Overview : | Recombinant Tobacco Etch Virus Protease (rTEV) contains 231 amino acids with N-terminal His tagged was expressed in E. coli. A fully biologically active molecule, rTEV has a molecular mass of 28.4 kDa and is obtained by proprietary chromatographic techniques. |
- Specification
- Gene Information
- Related Products
Description : | Tobacco Etch Virus Protease is a highly site-specific cysteine protease that is found in the Tobacco Etch Virus (TEV). The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) [ENLYFQ(G/S)] and cleavage occurs between the Gln and Gly/Ser residues, The most commonly used sequence is ENLYFQG. The protease is used to cleave affinity tags from fusion proteins. The optimal temperature for cleavage is 30 centigrade; also it can be used at temperature as low as 4 centigrade. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin. |
Source : | E. coli |
Species : | TEV |
Tag : | N-His |
Form : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Bio-activity : | 6 IU/μL |
Molecular Mass : | 28.4 kDa, observed by reducing SDS-PAGE. |
Protein Length : | 231 |
Purity : | > 95% by SDS-PAGE analyses. |
Unit Definition : | One unit of TEV protease cleaves > 85% of 3 μg of control substrate in 1 hour at pH 8.0 at 30 centigrade. |
Storage : | Recombinant Tobacco Etch Virus Protease (rTEV) remains stable up to 1 year at -20 centigrade from date of receipt. Please avoid freeze-thaw cycles. |
Storage Buffer : | Sterile liquid solution contains 50mM Tris, 5mM DTT, 50% glycerol, pH7.5. |
Official Symbol : | Protease |
Synonyms : | Protease |
Official Symbol : | Protease |
Synonyms : | Protease |
Products Types
◆ Recombinant Protein | ||
Protease-75T | Active Recombinant TEV Protease (AA 5-236), N-GFP and C-His-Tagged | +Inquiry |
Protease-92 | Recombinant Protease | +Inquiry |
Protease-01 | Active Recombinant Human Protease Protein, 6×His tagged | +Inquiry |
Protease-23T | Active Recombinant TEV Protease, His-tagged | +Inquiry |
◆ Native Protein | ||
Protease-20S | Active Native Streptomyces griseus Protease | +Inquiry |
◆ Assay kits | ||
Kit-2129 | HIV-1 Protease Fluorometric Activity Assay Kit | +Inquiry |
Kit-0720 | Protease Activity Fluorometric Assay Kit | +Inquiry |
Kit-0721 | Protease Fluorometric Assay Kit | +Inquiry |
Kit-1077 | HRV 3C Protease Activity Assay Kit (Colorimetric) | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
0
Inquiry Basket