"Ascorbic Acid" Related Products

Ascorbic Acid Colorimetric Assay Kit II (FRASC)

Cat.No.: Kit-1120
Description: Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent and an immune stimulant and is present in a wide variety of biological specimens. Due to the presence of a variety of other antioxidants in biological samples such as serum, most ascorbic acid assays show strong interference. FRASC Assay Kit provides a rapid, simple, and sensitive means of detecting ascorbic acid in biological samples such as serum and other body fluids, tissue and cell extracts, growth media and food products. In this assay, Fe;; is reduced to Fe2; by any antioxidants present. The ferrous iron is chelated with a colorimetric probe to produce a product with a strong absorbance band which can be monitored between 545-600 nm. The addition of ascorbate oxidase to parallel samples removes any ascorbate present leaving a background value which is subtracted from the total to give ascorbate content. The assay can detect 0.2 to 20 nmol of ascorbic acid in various samples.
Applications: The assay can detect 0.2 to 20 nmol of ascorbic acid in various samples.
Storage: -20°C
Shipping: Gel Pack
Size: 100 assays
Kit Components: FRASC Buffer; Ascorbic Acid Probe; FeCl3 solution; Ascorbate Oxidase (lyophilized); Ascorbic Acid Standard (20 μmole)
Target Species: Mammalian
Detection method: Absorbance (545-600 nm)
Features & Benefits: Simple procedure; takes ~ 30 minutes to complete the assay; Fast and convenient

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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