"ATF2" Related Products

Expand
Expand

ATF2 (pT69/pT71) Transcription Factor Assay Kit

Cat.No.: Kit-0111
Product Overview: ATF2 (pT69/pT71) Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double-stranded DNA (dsDNA) sequence containing the cAMP-response element (CRE) is immobilized onto the wells of a 96-well plate. ATF2 contained in a nuclear extract, binds specifically to the CRE. The activated ATF2 transcription factor complex is detected by addition of a specific primary antibody directed against the phospho-Thr(pT69/pT71) epitope on ATF2. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.
Size: 96 tests
Description: ATF2 (Activating Transcription Factor 2) is a sequence-specific DNA-binding protein belonging to the bZIP family of transcription factors that bind with high affinity to CRE (cAMP-responsive element) in the promoter region of target genes. ATF2 mediates both transcription and DNA damage control through its phosphorylation/ activation in response to inflammatory cytokines and other cellular stress factors.
Applications: Functional Studies more details.
Target Species: Reacts with: Human; Predicted to work with: all Mammals
Kit Components: Components: 96 tests; 96-Well Plate Cover: 1 unit; Polysorbate 20: 1 vial; TF ATF2 (Phospho-Thr69,71) 96-Well Strip Plate: 1 unit; TF ATF2 (Phospho-Thr69,71) Competitor dsDNA: 1 vial; TF ATF2 (Phospho-Thr69,71) Positive Control: 1 vial; TF ATF2 (Phospho-Thr69,71) Primary Antibody: 1 vial; Transcription Factor Antibody Binding Buffer (10X): 1 x 3ml; Transcription Factor Binding Assay Buffer (4X): 1 x 3ml; Transcription Factor Developing Solution: 1 x 12ml; Transcription Factor Goat Anti-Mouse HRP Conjugate: 1 vial; Transcription Factor Reagent A: 1 x 120µl; Transcription Factor Stop Solution: 1 x 12ml; Wash Buffer Concentrate (400X): 1 x 5ml
Compatible Sample Types: Nuclear Extracts

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.