cAMP Direct Immunoassay Detection Kit (Fluorometric)
Cat.No. : | Kit-0866 |
Product Overview : | cAMP Direct Immunoassay Detection Kit (Fluorometric) provides a sensitive method for detecting adenylate cyclase activity in biochemical or cell-based assay systems. Compared to other ELISA cAMP assay kits, this kit eliminates the tedious acetylation step. The kit uses AbRed Indicator as a fluorimetric substrate to quantify the HRP activity. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. The fluorescent product formed is proportional to the activity of HRP-cAMP conjugate. This kit is based on the competition between HRP-labeled cAMP and free cAMP for a fixed number of cAMP antibody binding sites. HRP-cAMP is displaced from the HRP-cAMP/anti-cAMP antibody complex by unlabeled free cAMP. In the absence of cAMP, HRP-cAMP conjugate is bound to anti-cAMP antibody exclusively. However, the unlabeled free cAMP in the test samples competes for anti-cAMP antibody with the HRP-cAMP antibody conjugate, therefore inhibits the binding of HRP-cAMP to anti-cAMP antibody. |
- Specification
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Size : | 1 x 96 tests |
Applications : | Functional Studies more details |
Storage : | Store at +4°C. |
Products Types
◆ Recombinant Protein | ||
CAMP-3377C | Recombinant Cutibacterium acnes CAMP protein(Met1-Pro275), His-tagged | +Inquiry |
CAMP-0346H | Recombinant Human CAMP Protein, GST-Tagged | +Inquiry |
Camp-661M | Recombinant Mouse Camp Protein, His/GST-tagged | +Inquiry |
Camp-360M | Recombinant Mouse Camp Protein, His-tagged | +Inquiry |
Camp-909M | Recombinant Mouse Camp Protein, MYC/DDK-tagged | +Inquiry |
◆ Lysates | ||
CAMP-7871HCL | Recombinant Human CAMP 293 Cell Lysate | +Inquiry |
◆ Assay kits | ||
Kit-0149 | cAMP Direct Immunoassay Kit | +Inquiry |
Kit-0148 | cAMP Activity Assay Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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