"Cell" Related Products

Cell Transformation Assay Kit (Colorimetric)

Cat.No.: Kit-1051
Description: The transformed cells can proliferate without attaching to surface. Anchorage independent cell growth is the hallmark of cell transformation. The Soft-Agar Assay is a traditional method for screening cell transformation in vitro. However, this method is lengthy (3-4 weeks incubation), laborious (counting colonies) and inconsistent (due to subjective counting). Cell Transformation Assay is faster, stable, and more sensitive than the traditional soft-agar assay. The kit is based on the conversion of the tetrazolium salt (WST-8) to formazan by cellular mitochondrial dehydrogenases. The generated signal is directly proportional to the number of living cells. This one-step method is non-radioactive and simple (just add-and-read, does not require harvesting cells, and solubilization steps). The assay is high-throughput adaptable and has wide linear range from 10000-400000 cells. The entire Cell Transformation Assay can be finished within 7-8 days.
Applications: Measure cell transformation in response to stimuli;
Screen and characterize compounds that influence cell transformation
Storage: -20°C
Shipping: Gel Pack
Size: 100 assays
Kit Components: Agarose Powder DMEM Solution (10X); Staining Solution; WST Reagent; Electro Coupling Solution (ECS)
Target Species: Mammalian cells
Detection method: Colorimetric (OD 450 nm)
Features & Benefits: Highly sensitive fluorometric method to measure cell transformation in response to a variety of biochemical stimuli;
Simple & High throughput-adaptable;
Reproducible, Quantitative tool for screening, studying, and characterizing compounds that affect cell transformation

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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