Recombinant Human Glutathione S-transferase Mu 4, His-tagged

• Cat.No.: GSTM4-780H
• Product Overview: Recombinant human GSTM4 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW =27.7kDa (238aa) confirmed by MALDI-TOF.

Specification

• Cat. No.: GSTM4-780H
• Description: Glutathione S-transferase Mu 4 isoform 1, also known as GSTM4, is member of the glutathione s-transferase (GST) family of proteins. There are eight families of GST proteins, namely alpha, kappa, mu, omega, pi, sigma, theta and zeta, each of which is composed of proteins that have a variety of functions throughout the cell. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione.
• Source: E.coli.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MSMTLGYWDI RGLAHAIRLL LEYTDSSYEE KKYTMGDAPD YDRSQWLNEK FKLGLDFPNL PYLIDGAHKI TQSNAILCYI ARKHNLCGET EEEKIRVDIL ENQAMDVSNQ LARVCYSPDF EKLKPEYLEE LPTMMQHFSQ FLGKRPWFVG DKITFVDFLA YDVLDLHRIF EPNCLDAFPN LKDFISRFEG LEKISAYMKS SRFLPKPLYT RVAVWGNK
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Publication:
  Maresin conjugates in tissue regeneration biosynthesis enzymes in human macrophages (2016)
  Biosynthesis of prostaglandin 15dPGJ2 -glutathione and 15dPGJ2-cysteine conjugates in macrophages and mast cells via MGST3 (2022)
  Biosynthetic metabolomes of cysteinyl-containing immunoresolvents (2019)

Gene Information

• Gene Name: GSTM4 glutathione S-transferase mu 4 [ Homo sapiens ]
• Synonyms: GSTM4; glutathione S-transferase mu 4; GTM4; GSTM4-4; MGC9247; MGC131945; GST-Mu2; GTS-Mu2; GST class-mu 4; glutathione S-transferase M4; glutathione S-aryltransferase M4; glutathione S-alkyltransferase M4; glutathione S-aralkyltransferase M4; S-(hydroxyalkyl)glutathione lyase M4; EC 2.5.1.18
• Gene ID: 2948
• mRNA Refseq: NM_000850
• Protein Refseq: NP_000841
• MIM: 138333
• UniProt ID: Q03013
• Chromosome Location: 1p13.3
• Pathway: Drug metabolism - cytochrome P450; Glutathione metabolism; Metabolism of xenobiotics by cytochrome P450
• Function: glutathione transferase activity; transferase activity