Recombinant Human Peroxiredoxin 2, His-tagged

• Cat.No.: PRDX2-503H
• Product Overview: Recombinant human PRDX2 protein with N-terminal His tag was expressed in an Baculovirus infectedSf9 cellexpression system, MW=22.7 kDa.

Specification

• Cat. No.: PRDX2-503H
• Description: Peroxiredoxin-2 is a protein that in humans is encoded by the PRDX2 gene. This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. The crystal structure of this protein has been resolved to 2.7 angstroms. Transcript variants encoding distinct isoforms have been identified for this gene.
• Source: Sf9 cell.
• Purity: > 95%.
• Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
• Specific Activity: 149 pmol/min/ug. 3.45 μg of PRDX1 was incubated in 50 mM Hepes (pH 7.0) containing 200 μM NADPH, 3 μM thioredoxin, and 1.5 μM thioredoxin reductase. The reaction mixture was incubated at 30 °C for 5 min, followed by the addition of 0.22 mM H2O2. NADPH oxidation was monitored (fluorescence decrease) for the next 10 min.
• Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: >6 months at –80°C.

Gene Information

• Gene Name: PRDX2 peroxiredoxin 2 [ Homo sapiens ]
• Synonyms: peroxiredoxin 2; PRP; TSA; PRX2; NKEFB; PRXII; TDPX1; MGC4104; PRDX2; torin; EC 1.11.1.15; thioredoxin peroxidase 1; thiol-specific antioxidant 1; natural killer-enhancing factor B; thiol-specific antioxidant protein; natural killer cell-enhancing factor B; hioredoxin-dependent peroxide reductase 1; Natural killer cell-enhancing factor B; Thioredoxin peroxidase 1; NKEF-B; Thioredoxin-dependent peroxide reductase 1
• Gene ID: 7001
• mRNA Refseq: NM_005809
• Protein Refseq: NP_005800
• MIM: 600538
• UniProt ID: P32119
• Chromosome Location: 19p13.2
• Function: antioxidant activity; oxidoreductase activity; thioredoxin peroxidase activity

Reviews

02/01/2022

I am eager to embark on this scientific journey, empowered by the high-quality PRDX2 protein and the manufacturer's unparalleled assistance.

12/16/2020

The manufacturer's prompt and reliable technical support serves as an invaluable resource, reinforcing my confidence in the PRDX2 protein's effectiveness and enabling smooth progress in my experiments.

11/01/2017

Its remarkable purity and functional attributes make it an optimal choice for my research, ensuring accurate and reliable outcomes.

Q&As

How does PRDX2 contribute to the field of cancer research and treatment?

PRDX2's role in maintaining cellular redox balance implicates it in cancer progression. Modulating PRDX2 levels may offer avenues for developing novel cancer therapies.

Are there any side effects associated with targeting PRDX2 for therapeutic purposes?

As with any targeted therapy, potential side effects need thorough investigation. Modulating PRDX2 levels could impact normal cellular functions, and careful assessment is required.

Is there evidence supporting the use of PRDX2 as a prognostic marker in certain diseases?

Some studies suggest that PRDX2 levels may have prognostic value in diseases associated with oxidative stress, providing insights into disease progression.

Can PRDX2 be a target for drug development, and what challenges might be associated with this?

Yes, PRDX2 is a potential drug target, but challenges include developing specific modulators and understanding the protein's complex regulatory mechanisms.

Are there any specific diagnostic tests or biomarkers related to PRDX2 protein levels?

Research suggests that altered levels of PRDX2 could serve as a biomarker in diseases associated with oxidative stress, offering potential diagnostic applications.