Active Recombinant Human TSHB protein

• Cat.No.: TSHB-105H
• Product Overview: Recombinant Human TSHB(Phe21-Val138)&TSH alpha(Ala25-Ser116) was expressed in CHO.

Specification

• Source: CHO
• Species: Human
• Predicted N Terminal: Ala25 (TSH alpha ) & Phe21 (TSH beta )
• Form: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce cAMP accumulation in HEK293 human embryonic kidney cells transfected with human TSH R. Morgenthaler, N.G. et al. (1998) Horm. Metab. Res. 30:162. The ED50 for this effect is typically 0.25-1.5 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 10.2 (TSH alpha ), 13.5 (TSH beta ) kDa;
  SDS-PAGE: 15-29 kDa, reducing conditions.
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: TSHB thyroid stimulating hormone, beta [ Homo sapiens ]
• Official Symbol: TSHB
• Synonyms: TSHB; thyroid stimulating hormone, beta; thyrotropin subunit beta; thyrotropin beta chain; thyrotropin beta subunit; thyroid-stimulating hormone subunit beta; TSH-B; TSH-BETA
• Gene ID: 7252
• mRNA Refseq: NM_000549
• Protein Refseq: NP_000540
• MIM: 188540
• UniProt ID: P01222