Recombinant Human TSHB(Phe21-Val138)&TSH alpha(Ala25-Ser116) was expressed in CHO.
Predicted N Terminal:
Ala25 (TSH alpha ) & Phe21 (TSH beta )
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Measured by its ability to induce cAMP accumulation in HEK293 human embryonic kidney cells transfected with human TSH R. Morgenthaler, N.G. et al. (1998) Horm. Metab. Res. 30:162. The ED50 for this effect is typically 0.25-1.5 ng/mL.
Predicted Molecular Mass: 10.2 (TSH alpha ), 13.5 (TSH beta ) kDa;
SDS-PAGE: 15-29 kDa, reducing conditions.
<1.0 EU per 1 μg of the protein by the LAL method.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.