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Recombinant Human TSHB protein

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Cat.No.:
TSHB-105H
Product Overview:
Recombinant Human TSHB(Phe21-Val138)&TSH alpha(Ala25-Ser116) was expressed in CHO.
Source:
CHO
Species:
Human
Predicted N Terminal:
Ala25 (TSH alpha ) & Phe21 (TSH beta )
Form:
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Bio-activity:
Measured by its ability to induce cAMP accumulation in HEK293 human embryonic kidney cells transfected with human TSH R. Morgenthaler, N.G. et al. (1998) Horm. Metab. Res. 30:162. The ED50 for this effect is typically 0.25-1.5 ng/mL.
Molecular Mass:
Predicted Molecular Mass: 10.2 (TSH alpha ), 13.5 (TSH beta ) kDa;
SDS-PAGE: 15-29 kDa, reducing conditions.
Endotoxin:
<1.0 EU per 1 μg of the protein by the LAL method.
Purity:
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage:
Avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution:
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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