Recombinant Bovine Fibroblast Growth Factor 2 (basic)

• Cat.No.: FGF2-1182B
• Product Overview: Recombinant bovine FGF2is a single, non-glycosylated polypeptide chain containing 172 amino acids.

Specification

• Cat. No.: FGF2-1182B
• Description: FGF2 is asingle-chain polypeptide growth factor that plays a significant role in theprocess of wound healing and is a potent inducer of angiogenesis. Severaldifferent forms of the human protein exist ranging from 18-24 kDa in size dueto the use of alternative start sites within the FGF-2 gene. It has a 55percent amino acid residue identity to FGF-1 and has potent heparin-bindingactivity.
• Source: E. coli
• Species: Bovine
• Form: Lyophilized from asterile filtered solution in PBS, pH 7.4+4% mannitol.
• Molecular Weight: 18.5 kDa
• Purity: > 97% by SDS-PAGEand HPLC analyses
• Endotoxin Level: < 0.1 ng/µg ofFGF2
• Biological Activity: Calculated by thedose dependant proliferation of BALB/c 3T3 cells, the ED50 is <1.0 ng/ml.
• Specific Activity: 1 x 106Units/mg
• Sequences: MTMITNSSSVPGDPLESMAA GSITTLPALP EDGGSGAFPP GHFKDPKRLY CKNGGFFLRI HPDGRVDGVR EKSDPHIKLQLQAEERGVVS IKGVCANRYL AMKEDGRLLA SKCVTDECFF FERLESNNYN TYRSRKYSSW YVALKRTGQYKLGPKTGPGQ KAILFLPMSA KS
• Reconstitution: Centrifuge vialprior to opening. Add steriledistilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL.Further dilutions should be made in appropriate buffered solutions.
• Storage: Stable at 2-8°C, butbest kept desiccated -20°C. Upon reconstitution, stable for up to 1 week at2-8°C. For longer term, store in working aliquots below -20°C. Avoid repeatedfreeze/thaw cycles.
• OfficialSymbol: FGF2

Gene Information

• Gene Name: FGF2 fibroblast growth factor2 (basic) [ Bos taurus ]
• Synonyms: FGF2; fibroblastgrowth factor 2 (basic); heparin-binding growth factor 2; BFGF; HBGF-2; basicfibroblast growth factor
• Gene ID: 281161
• mRNA Refseq: NM_174056
• Protein Refseq: NP_776481
• UniProt ID: P03969
• Chromosome Location: 17
• Pathway: Downstream signalingof activated FGFR; FGFR ligand binding and activation; FGFR1 ligand bindingand activation; FGFR1b ligand binding and activation; FGFR1c ligand bindingand activation; FGFR2 ligand binding and activation; FGFR2c ligand bindingand activation; FGFR3 ligand binding and activation; FGFR4 ligand binding andactivation; FRS2-mediated cascade; IRS-mediated signalling; Insulin receptorsignalling cascade; MAPK signaling pathway; Melanoma
• Function: growth factoractivity; heparin binding; voltage-gated calcium channel activity

Reviews

07/14/2022

The technical expert communicated with me in time to understand the progress of my experiment and gave me suggestions for improvement.

02/04/2022

The purchased protein is suitable for WB experiments and I am satisfied with the results.

Q&As

What is the biological function of FGF2?

FGF2 promotes cell migration and angiogenesis and regulates bone homeostatic balance.

Where is FGF2 mainly expressed?

FGF2 is widely expressed in the nervous system.

In which tissues of sheep is FGF2 primarily expressed?

FGF2 is mainly expressed in tissues such as mammary glands and ovaries of sheep.

What protein does FGF2 share high homology with?

FGF1 has a high degree of homology with FGF2.

What diseases is FGF2 involved in?

FGF2 is involved in diseases such as cardiovascular disease, breast disease and bone homeostasis.

How does FGF2 regulate bone homeostasis?

FGF2 negatively regulates bone homeostasis.