Recombinant Human EXOSC10 fused with His tag was expressed in Insect cells.
EXOSC10 is a nucleolar protein and represents one crucial component of the so-called "PM/Scl complex" or "nuclear exosome", which is an RNA-processing complex consisting of a number of exoribonucleases (not to be confused with certain multivesicular bodies which are shed by cultured cells into the culture fluid and are also called exosomes).
EXOSC10 is supplied at a concentration of 0.8mg/ml in 20mM HEPES, pH-7.5, 100mM NaCl, 0.01mM EDTA, 0.02% SDS.
Greater than 70% as determined by SDS-PAGE.
Western-Blot with monoclonal anti-hexa-His-tag antibody & polymyositis patient sera.
Coating concentration: 0.4-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Store at 4 centigrade if entire vial will be used within 2-4 weeks.Store, frozen at -20 centigrade for longer periods of time.Avoid multiple freeze-thaw cycles.