Recombinant Human Insulin-Like Growth Factor 2 (somatomedin A)

• Cat.No.: IGF2-5430H
• Product Overview: Recombinant humanIGF2 was expressed in E. coli.

Specification

• Cat. No.: IGF2-5430H
• Description: Human Insulin-likeGrowth Factor-II (IGF-II) is a single chain 67 amino acid polypeptide thatshares structural homology with insulin and human IGF-I. Human IGF-II iswidely distributed in many tissues throughout the body and acts on numerouscell types through the Type 1 IGF-I receptor. IGF-II also binds specificallyto the IGF-II / cation-independent mannose-6-phosphate receptor as well asthe family of six IGF binding proteins, but the physiological function ofIGF-II as a result of all these interactions has not been clearlyestablished.
• Source: E. coli
• Species: Human
• Purity: Greater than 95% (byHPLC and N-terminal sequence analysis).
• Molecular Weight: 7.649kDa-homogeneity confirmed by Mass Spectometry.
• Endotoxin Level: Less than 0.1 EU/ug
• Biological Activity: Type 1 IGF receptorbinding assay: ED50>20 ng/ml. Type 2 IGF receptor binding assay: ED50<70ng/ml. IGF binding protein assay: ED50<10 ng/ml. Stimulation of proteinsynthesis in rat L6 myoblasts: ED50 Less than 150 ng/ml.
• State and appearance: Lyophilized whitepowder.
• N-terminal sequenceanalysis: 7 residues Greaterthan 95% single sequence.
• Reconstitution: Refer to Protocol1000: Handling of GroPep IGF-I, IGF-II and IGF Analogs.
• Storage: At least 2 years at2-4°C (lyophilized).
• OfficialSymbol: IGF2

Gene Information

• Gene Name: IGF2 insulin-like growth factor2 (somatomedin A) [ Homo sapiens ]
• Synonyms: IGF2; insulin-likegrowth factor 2 (somatomedin A); IGF-II; PP9974; C11orf43; FLJ22066; FLJ44734;insulin-like growth factor II;somatomedin-A; OTTHUMP00000011012; OTTHUMP00000011013; OTTHUMP00000011015;OTTHUMP00000011018; OTTHUMP00000011157; OTTHUMP00000011158;OTTHUMP00000220677; insulin-like growth factor type 2
• Gene ID: 3481
• mRNA Refseq: NM_000612
• Protein Refseq: NP_000603
• MIM: 147470
• UniProt ID: P01344
• Chromosome Location: 11p15
• Pathway: Apoptosis;Diabetes pathways; Endochondral Ossification; Hedgehog Signaling Pathway;Regulation of Insulin-like Growth Factor (IGF) Activity by Insulin-likeGrowth Factor Binding Proteins (IGFBPs)
• Function: growthfactor activity; hormone activity; insulin receptor binding; insulin-likegrowth factor receptor binding; protein binding; protein serine/threoninekinase activator activity; receptor activator activity