Recombinant Human NAGK protein, His-tagged

• Cat.No.: NAGK-2959H
• Product Overview: Recombinant Human NAGK(1-344aa) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: NAGK (N-acetyl-D-glucosamine kinase) belongs to the eukaryotic-type N-acetylglucosamine kinase family. NAGK converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. It is a prominent salvage enzyme of amino sugar metabolism in mammals. NAGK has been shown to interact with STK16 and LNX1. Also it has ManNAc kinase activity.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200mM NaCl
• Molecular Mass: 39.8kDa (367aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSLRSLGLSLSG GDQEDAGRIL IEELRDRFPY LSESYLITTD AAGSIATATP DGGVVLISGT GSNCRLINPD GSESGCGGWG HMMGDEGSAYMAAIYGG VEGGGTRSEV LLVSEDGKIL AEADGLSTNH WLIGTDKCVE RINEMVNRAK RKAGVDPLVPWIAHQAVKIV FDSIDNLEAA PHDIGYVKQA MFHYFQVPDR LGILTHLYRD FDKCRFAGFC RKIAEGAQQG DPLSRYIFRK AGEMLGRHIVAVLPEIDPVL FQGKIGLPIL CVGSVWKSWE LLKEGFLLAL TQGREIQAQN FFSSFTLMKL RHSSALGGAS LGARHIGHLL PMDYSANAIAFYSYTFS
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: NAGK N-acetylglucosamine kinase [ Homo sapiens ]
• Official Symbol: NAGK
• Synonyms: NAGK; N-acetylglucosamine kinase; N-acetyl-D-glucosamine kinase; GNK; glcNAc kinase; HSA242910
• Gene ID: 55577
• mRNA Refseq: NM_017567
• Protein Refseq: NP_060037
• MIM: 606828
• UniProt ID: Q9UJ70
• Chromosome Location: 2p24.3-p24.1
• Pathway: Amino sugar and nucleotide sugar metabolism, organism-specific biosystem; Amino sugar and nucleotide sugar metabolism, conserved biosystem; N-acetylglucosamine degradation II, organism-specific biosystem
• Function: ATP binding; N-acetylglucosamine kinase activity; N-acylmannosamine kinase activity; kinase activity; nucleotide binding; protein binding; transferase activity