Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Antibody > Monoclonal Antibodies > Mouse Anti-Human Acrosomal Vesicle Protein 1 Monoclonal Antibody

Mouse Anti-Human Acrosomal Vesicle Protein 1 Monoclonal Antibody

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Human(6)
Mouse(5)
Rat(3)
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His(3)
Cat.No.:
CAB11477MH
Product Overview:
Mouse monoclonal antibody to human acrosomal vesicle protein 1.
Antigen Description:
Acrosomal protein SP-10, also known as Acrosomal vesicle protein 1 and ACRV1, is a testis-specific, differentiation antigen, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. Regulation of cell type-specific gene transcription is central to cellular differentiation and development. During spermatogenesis, a number of testis-specific genes are expressed in a precise spatiotemporal order. The longest transcript of ACRV1 / SP-10 is the most abundant, comprising 53-72% of the total acrosomal vesicle protein 1 messages; the second largest transcript comprises 15-32%; the third and the fourth largest transcripts account for 3.4-8.3% and 8.7-12.5%, respectively; and the remaining transcripts combined account for< 1% of the total acrosomal vesicle protein 1 message. acrv1 sp-10 is a testis-specific acrosomal protein that has been detected in several species including humans. it may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine immunogen for humans. acrv1 sp-10 may be involved in sperm-zona binding or penetration. it is also a intra-acrosomal protein that is considered to be a vaccine candidate for>
Specificity:
Human ACRV1. No cross-reactivity with human cell lysate ( 293 cell line ) in ELISA.
Immunogen:
Recombinant Human ACRV1 Protein
Host:
Mouse
Isotype:
Mouse IgG1
Species:
Human
Clone:
4E3G11
Purification:
Protein A affinity chromatography
Applications:
Western Blot; ELISA
Dilution:
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect Human ACRV1 in WB. Using a DAB detection system, the detection limit for Human ACRV1 is approximately 20 ng/lane under non-reducing conditions and reducing conditions.Direct ELISA: This antibody can be used at 0.5 - 1 μg with the appropriate secondary reagents to detect Human ACRV1. The detection limit for Human ACRV1 is approximately 0.0195 ng/well.
Preparation:
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human ACRV1. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Format:
0.2 μm filtered solution in PBS with 5% trehalose
Storage:
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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