Rabbit Monoclonal Antibody to Human ErbB2 / HER2, FITC-Conjugated
ErbB2, also known as CD340 and HER2, is a type I membrane glycoprotein. It belongs to the epidermal growth factor (EGF) receptor family. ErbB2 cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, this receptor forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. ErbB2 mediates signalling pathways which involve mitogen-activated protein kinase and phosphatidylinositol-3 kinase, this receptor plays an key role in development, cell proliferation and differentiation. ErbB2 is widely expressed in epithelial cells, and amplification and/or overexpression of the this gene has been reported associated with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate and ovarian cancers.
Human ErbB2 / HER2 / CD340
Recombinant human ErbB2 protein
Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human ErbB2 in WB. Using a DAB detection system, the detection limit for Human ErbB2 is approximately 1 ng/lane under non-reducing conditions.
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human ErbB2 / HER2 extracellular domain and conjugated with FITC under optimum conditions, the unreacted FITC was removed
Aqueous solution containing 0.5% BSA and 0.1% sodium azide
5 μl/Test, 0.2 mg/ml
This antibody is stable for 12 months from date of receipt when stored at 2-8 °C. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Analysis of anti-ErbB2 on SK-BR3 cells: SK-BR3 cells were detached using 1X trypsin, washed, then stained with FITC Rabbit anti-Human ErbB2.