| Antigen Description : |
Plasminogen activator, urokinase, also known as PLAU and uPA, is a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. The human PLAU is initially synthesized as 431 amino acid precursor with a N-terminal signal peptide (20 residues). The single chain molecule is processed into a disulfide-linked two-chain molecule of different molecular weights. Two forms of the A chain exist, starting at Ser21 (the long form) and Lys156 (the short form). The long and short A chains are unique to the high and low molecular weight forms, respectively. The long A chain contains an EGF-like domain, responsible for binding of the PLAU receptor. The B chain corresponds to the catalytic domain. PLAU is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium. A specific polymorphism in PLAU gene is implicated in late-onset Alzheimer disease and also with decreased affinity for fibrin-binding. |
| Specificity : |
Human uPA / PLAU. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA. |
| Immunogen : |
Recombinant human uPA protein |
| Host : |
Mouse |
| Isotype : |
MouseIgG1 |
| Species : |
Human |
| Clone : |
2B3H5H12 |
| Applications : |
Western blot |
| Dilution : |
This antibody can be used at 1-2ug/mL with the appropriate secondary reagents to detect A chain and full-length of uPA in WB. Using a DAB detection system, the detection limit for uPA is approximately 2ng/lane and 100ng/lane under non-reducing conditions and reducing conditions. |
| Preparation : |
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human uPA / PLAU. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Format : |
0.2 μm filtered solution in PBS with 5% trehalose |
| Storage : |
This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
