Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Antibody > Monoclonal Antibodies > Mouse Monoclonal Antibody to Human Plasminogen Activator, Urokinase

Mouse Monoclonal Antibody to Human Plasminogen Activator, Urokinase

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Cat.No.:
CAB11591MH
Product Overview:
Mouse Monoclonal Antibody to Human Plasminogen Activator, Urokinase
Antigen Description:
Plasminogen activator, urokinase, also known as PLAU and uPA, is a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. The human PLAU is initially synthesized as 431 amino acid precursor with a N-terminal signal peptide (20 residues). The single chain molecule is processed into a disulfide-linked two-chain molecule of different molecular weights. Two forms of the A chain exist, starting at Ser21 (the long form) and Lys156 (the short form). The long and short A chains are unique to the high and low molecular weight forms, respectively. The long A chain contains an EGF-like domain, responsible for binding of the PLAU receptor. The B chain corresponds to the catalytic domain. PLAU is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium. A specific polymorphism in PLAU gene is implicated in late-onset Alzheimer disease and also with decreased affinity for fibrin-binding.
Specificity:
Human uPA / PLAU. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
Immunogen:
Recombinant human uPA protein
Host:
Mouse
Isotype:
MouseIgG1
Species:
Human
Clone:
2B3H5H12
Applications:
Western blot
Dilution:
This antibody can be used at 1-2ug/mL with the appropriate secondary reagents to detect A chain and full-length of uPA in WB. Using a DAB detection system, the detection limit for uPA is approximately 2ng/lane and 100ng/lane under non-reducing conditions and reducing conditions.
Preparation:
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human uPA / PLAU. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Format:
0.2 μm filtered solution in PBS with 5% trehalose
Storage:
This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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