Mouse Monoclonal Antibody to Human Serine Peptidase Inhibitor, Clade D, Member 1
SerpinD1, also known as heparin cofactor II (HCII),is a member of Serpin superfamily of the serine proteinase inhibitors. HCII is a glycoprotein in human plasma that inhibits thrombin and chymotrypsin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans(GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. Interestingly, a C-terminal His-tagged recombinant HCII exhibits enhanced activity of thrombin inhibition. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis.
Human SerpinD1 / HC2. No cross-reactivity in ELISA with Human SerpinA3; Human SerpinC1; Human SerpinI1; Mouse SerpinD1; Mouse SerpinF1; Mouse SerpinF2; Human cell lysate (293 cell line)
Recombinant Human SerpinD1 / HC2 Protein
Western blot; ELISA
Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human SerpinD1 in WB. Using a DAB detection system, the detection limit for Human SerpinD1 is approximately 2 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human SerpinD1. The detection limit for Human SerpinD1 is approximately 0.31 ng/well.
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human SerpinD1 / HC2. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.