Rabbit Monoclonal Antibody to Human Ubiquitin Carboxyl-Terminal Esterase L1
Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqutinating enzyme with important functions in recycling of ubiquitin. Regulated proteolysis by the ubiquitin pathway has been implicated in control of the cell cycle, transcriptional activation, cell fate and growth, and synaptogenesis. The ubiquitin-proteasome system is involved in synaptic plasticity and is proposed to be part of a molecular switch that converts short-term synaptic potentiation to long-term changes in synaptic strength. UCHL1 is found in neuronal cell bodies and processes throughout the neocortex (at protein level). It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. UCHL1 is weakly expressed in ovary. UCHL1 is a ubiquitin-protein hydrolase. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. UCHL1 dysfunction has been associated with neurodegeneration in Parkinsons, Alzheimers, and Huntingtons disease patients. Reduced UCHL1 function may jeopardize the survival of CNS neurons.
Human UCHL1 / PGP9.5. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
Recombinant human UCHL1 protein
Western blot; ELISA
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human UCHL1 in WB. Using a DAB detection system, the detection limit for human UCHL1 is approximately 0.5 ng/lane under non-reducing conditions and 0.25 ng/lane under reducing conditionsDirect ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human UCHL1. The detection limit for human UCHL1 is approximately 0.00245 ng/well
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human UCHL1
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.