A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexahistidine-tag, 6xHis-tag, and by the trademarked name His-tag (registered by EMD Biosciences).
A synthetic peptide HHHHHH coupled to KLH.
The antibody has been tested by Western blot. Recommended dilution is 1:5000, Optimal dilutions/concentrations should be determined by the end user.
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Can be stored at +4°C. For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.