| Cat. No. : |
RETN-63H |
| Overview : |
Human Resistin Recombinant Protein was expressed in E. coli with 9.9 kDa protein containing 93 amino acid residues and purified by Two-step procedure using size exclusion chromatography before and after refolding. |
| Description : |
Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family, and is also described as ADSF (Adipose Tissue- Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Human resistin contains 108 amino acids as a prepeptide, and its hydrofobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides, which are disulfide-linked via Cys26. |
| Source : |
E. coli. |
| Species : |
Human |
| Predicted MolecularMass : |
9.9 kDa |
| Formulation : |
Recombinant Human Resistin was lyophilized from a concentrated (1mg/ml) solution containing 0.03% NaHCO3. |
| Purity : |
Greater than 98% as determined by SDS-PAGE. |
| Specificity : |
The amino acid sequence of the Resistin Human Recombinant is homologous to the amino acid sequence of the Resistin Human without signal sequence. |
| Solubility : |
Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely. |
| Bioactivity : |
The biological activity was evidenced by the Resistin ability to prevent insulin-stimulated uptake of deoxy glucose in several cell lines at 10 ng/ml concentration corresponding to a Specific Activity of 100,000IU/mg. |
| Storage & Stability : |
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| OfficialSymbol : |
RETN |
