Active Recombinant Human PDE4A, GST-tagged

• Cat.No.: PDE4A-1527H
• Product Overview: Recombinant human PDE4A (332-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: PDE4A is a member of the phosphodiesterase family of proteins that play a critical role in regulating intracellular levels of cAMP. In vitro phosphorylation of PDE4A by the PKA-catalytic subunit increases the enzymes sensitivity to Mg(2+), leading to a 4-fold increase in cAMP hydrolysis without affecting the Km. PDE4 is widely expressed in brain tumors and promotes their growth and treatment with PDE4A inhibitor Rolipram overcomes tumor resistance and mediates tumor regression. PDE4A expression is altered in patients with bipolar disorder and is a new therapeutic avenue for treatment of this disorder.
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 1082 nmol/min/mg
• Molecular Mass: ~110 kDa
• Protein length: 332 aa-end
• Purity: >95%
• Applications: PDE Assay
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: PDE4A phosphodiesterase 4A, cAMP-specific [ Homo sapiens ]
• Official Symbol: PDE4A
• Synonyms: PDE4A; phosphodiesterase 4A, cAMP-specific; DPDE2, phosphodiesterase 4A, cAMP specific (dunce (Drosophila) homolog phosphodiesterase E2); cAMP-specific 3,5-cyclic phosphodiesterase 4A; phosphodiesterase E2 dunce homolog (Drosophila); phosphodiesterase isozyme 4; phosphodiesterase 4A, cAMP-specific (dunce; phosphodiesterase E2 dunce homolog, Drosophila; PDE4; DPDE2; PDE46
• Gene ID: 5141
• mRNA Refseq: NM_001111307
• Protein Refseq: NP_001104777
• MIM: 600126
• UniProt ID: P27815
• Chromosome Location: 19p13.2
• Pathway: DARPP-32 events, organism-specific biosystem; G Protein Signaling Pathways, organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; Morphine addiction, organism-specific biosystem; Morphine addiction, conserved biosystem; Opioid Signalling, organism-specific biosystem
• Function: 3,5-cyclic-AMP phosphodiesterase activity; hydrolase activity; metal ion binding; phosphoric diester hydrolase activity; protein binding

Reviews

12/05/2022

PDE4A requires less storage environment.

07/26/2022

The expression of PDE4A was stable.

02/08/2022

It has the advantage of easy operation in the expression system and can improve the expression efficiency.

Q&As

How effective are PDE4A inhibitors in the treatment of asthma?

The inhibitors have been used in the treatment of asthma, and studies have shown that they can significantly reduce the number of asthma attacks and the use of drug D.

How to introduce the structure of PDE4A?

This protein has multiple conserved regions and different subtypes, which is of great significance for the selective inhibition of drugs.

What is a selective PDE4A inhibitor?

Selective PDE4A inhibitors refer to drug molecules that target only the PDE4A protein and do not inhibit other PDE4 homologs.

What is the mechanism by which PDE4A regulates T cell-mediated autoimmune responses?

It plays an important role in the activation and regulation of T cells by regulating cAMP levels, and is involved in T cell-mediated autoimmune responses.

Are levels of PDE4A associated with sleep disturbances?

Studies have shown that PDE4A depletion may be related to sleep-wake behavior and time stress response, but its specific role needs to be further studied.

What is the expression pattern of PDE4A in T cells?

In T cells, PDE4A is mainly expressed in the cytoplasm and peripheral regions of the nuclear membrane.