Recombinant Human TRDMT1 , GST-tagged

• Cat.No.: TRDMT1-6969H
• Product Overview: Recombinant full-length human TRDMT1 (DNMT2) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: TRDMT1 is responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. TRDMT1 also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, TRDMT1 is distinct and highly conserved in its function among various species. The indirect sequence recognition is also a feature of eukaryotic DNA methyltransferases, which may have arisen from a DNMT2-like RNA methyltransferase. DNMT2 is involved in retrotransposon silencing and telomere integrity in somatic cells.
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~67 kDa
• Protein length: Full length
• Purity: >95%
• Applications: Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1 μg/μ

Gene Information

• Gene Name: TRDMT1 tRNA aspartic acid methyltransferase 1 [ Homo sapiens ]
• Official Symbol: TRDMT1
• Synonyms: TRDMT1; tRNA aspartic acid methyltransferase 1; DNA (cytosine 5 ) methyltransferase 2 , DNMT2; tRNA (cytosine(38)-C(5))-methyltransferase; RNMT1; DNA methyltransferase-2; DNA MTase homolog HsaIIP; tRNA (cytosine-5-)-methyltransferase; DNA (cytosine-5)-methyltransferase-like protein 2; DMNT2; DNMT2; PUMET; MHSAIIP
• Gene ID: 1787
• mRNA Refseq: NM_004412
• Protein Refseq: NP_004403
• MIM: 602478
• UniProt ID: O14717
• Chromosome Location: 10p15.1
• Function: DNA (cytosine-5-)-methyltransferase activity; DNA binding; RNA binding; methyltransferase activity; transferase activity