Mouse Anti-Bovine CD4 Hybridoma
Cat.No. : | CSC-H0173 |
- Specification
- Gene Information
- Related Products
- Download
Background : | CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. It was discovered in the late 1970s and was originally known as leu-3 and T4 (after the OKT4 monoclonal antibody that reacted with it) before being named CD4 in 1984. In humans, the CD4 protein is encoded by the CD4 gene. |
Cell Line Description : | The antibody is directed against B°CD4 antigen, the bovine equivalent of human CD4 found on helper/inducer T cells. |
Depositor : | Dr J Naessens, ILRAD, Nairobi, Kenya |
Immunogen : | Bovine alloreactive lymphocytes |
Immunological Donor : | BALB/c mouse spleen |
Fusion Species : | Mouse X Mouse Hybridoma |
Myeloma : | P3x63Ag8 |
Growth properties : | Suspension |
Propagation : | Complete growth medium: RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS) Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0 °C |
Subculturing : | Maintain cultures between 3-9x10^5 cells/ml; 5% CO2; 37 °C. |
Mycoplasma : | Mycoplasma Status: Negative (MycoAlert Kit) |
Reactivity : | Anti-CD4, bovine |
Isotype : | IgG2a |
Target : | CD4 |
Application : | Enumeration of bovine lymphocyte populations |
Storage : | Liquid nitrogen |
Safety Considerations : | The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the hybridoma.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with hybridoma.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach. |
Ship : | Dry ice |
Gene Name : | CD4 CD4 molecule [ Bos taurus ] |
Official Symbol : | CD4 |
Synonyms : | T-cell surface glycoprotein CD4; CD4 antigen; CD4 |
Gene ID : | 407098 |
mRNA Refseq : | NM_001103225 |
Protein Refseq : | NP_001096695 |
Chromosome Location : | 5 |
Pathway : | Antigen processing and presentation; Cell adhesion molecules (CAMs); Cytokines and Inflammatory Response (Bi°Carta); Hematopoietic cell lineage; Primary immunodeficiency; T cell receptor signaling pathway |
Products Types
◆ Lysates | ||
CD4-1865FCL | Recombinant Ferret CD4 cell lysate | +Inquiry |
CD4-947CCL | Recombinant Cynomolgus CD4 cell lysate | +Inquiry |
CD4-1905HCL | Recombinant Human CD4 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (3)
Ask a questionCD4 binds to glycoprotein GP120.
CD4 is mainly expressed in T cells and thymocytes.
CD4 is localized in the cell membrane.
Customer Reviews (3)
Write a reviewThe CD4 proteins purchased from your company is suitable for ELISA.
Protein products are put into experiments with good effect and reliable experimental results.
The technical expert will follow up on the progress of my experiments and provide constructive advice on the problems that arise in my experiments.
Ask a Question for All CD4 Products
Required fields are marked with *
My Review for All CD4 Products
Required fields are marked with *
Inquiry Basket