Rat Anti-Havcr1 Monoclonal Antibody
Tim-1 is highly expressed by activated CD4+ T cells, and acts as a positive/negative co-stimulatory molecule of T cell proliferation, cytokine production and tolerance abrogation, and is the receptor for Tim-4, expressed on APCs. Tim-1 expression is greater on Th2 than Th1 cells, and studies have shown that interaction between Tim-1 on Th2 cells and Tim-4 on dendritic cells (DCs), enhances Th2 cell function, and have implicated Tim-1 as a critical player in the development of atopic disease, and in particular airway hypersensitivity.
It specifically recognises mouse Tim-1 (T-cell immunoglobulin mucin 1), a cell surface glycoprotein and member of the immunoglobulin superfamily, which was first identified as the hepatitis A virus cellular receptor (HAVCR), and is highly expressed in the liver (isoform Tim-1a) and the kidneys (isoform Tim-1b).
Full-length mouse Tim-1-Ig, containing both the IgV and mucin domains of Tim-1.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Reconstitute with 1.0 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial.
Prior to reconstitution store at +4°C. After reconstitution store at +4°C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
0.09% Sodium Azide (NaN3),1% Bovine Serum Albumin,5% Sucrose
12 months from date of reconstitution.