Active Recombinant Human CD22 protein, His-tagged
Cat.No. : | CD22-3946H |
Product Overview : | Recombinant Human CD22 protein(P20273-1)(Met1-Arg687), fused with His tag, was expressed in HEK293. |
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Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | Measured by its binding ability in a functional ELISA. Immobilized human CD22 at 0.02 μg/ml (100uL/well) can bind anti-human CD22 Mab with a linear range of 0.2-50 ng/ml. |
Molecular Mass : | The recombinant human CD22 consists of 679 amino acids and predictes a molecular mass of 76.6 kDa. As a result of glycosylation, it migrates as an approximately 108 kDa band in SDS-PAGE under reducing conditions. |
Protein Length : | Met1-Arg687 |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | ≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. |
Storage : | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Publication : |
Development and characterization of a camelid single‐domain antibody directed to human CD22 biomarker (2018)
Extremely low frequency magnetic field enhances expression of a specific recombinant protein in bacterial host (2022)
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Gene Name : | CD22 CD22 molecule [ Homo sapiens ] |
Official Symbol : | CD22 |
Synonyms : | CD22; CD22 molecule; CD22 antigen; B-cell receptor CD22; sialic acid binding Ig like lectin 2; SIGLEC 2; SIGLEC2; BL-CAM; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid binding Ig-like lectin 2; sialic acid-binding Ig-like lectin 2; SIGLEC-2; FLJ22814; MGC130020; |
Gene ID : | 933 |
mRNA Refseq : | NM_001185099 |
Protein Refseq : | NP_001172028 |
MIM : | 107266 |
UniProt ID : | P20273 |
Products Types
◆ Lysates | ||
CD22-1956HCL | Recombinant Human CD22 cell lysate | +Inquiry |
CD22-001MCL | Recombinant Mouse CD22 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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