Active Recombinant Human Integrin, Beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)

• Cat.No.: ITGB1-1484H
• Product Overview: TIMP1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 184 amino acids with a molecular weight of 20.7 kDa though on SDS-PAGE migrates at about 28kDa due to the glycosilation. The TIMP1 is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: ITGB1-1484H
• Description: TIMP1 is a member of the TIMP family. TIMP1 is an inducible glycoprotein produced by various cell types. The TIMP1 glycoprotein is a natural inhibitor of the matrix metalloproteinases, which a group of peptidases involved in degradation of the extracellular matrix. TIMP1 binds in a reversible mode to MMPs, with regions in the N-terminal domain binding to the MMP substrate-binding site.
• Source: Human
• Host: HEK293-F Cells.
• Form: The TIMP1 protein was lyophilized from a 0.2µm filtered solution containing no additives or preservatives.
• Purity: Greater than 95.0% as determined by SDS-PAGE
• Bio-activity: IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-(3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl)-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, and the peptide substrate concentration is 5µM.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized TIMP1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Amino acid sequence: CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA
• Storage: Lyophilized TIMP1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TIMP1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: ITGB1 integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) [ Homo sapiens ]
• Official Symbol: ITGB1
• Synonyms: ITGB1; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); FNRB; CD29; GPIIA; Fibronectin receptor subunit beta; MDF2; MSK12; VLA-BETA; VLAB; integrin beta-1; integrin VLA-4 beta subunit; very late activation protein, beta polypeptide; CD29 antigen; VLA-4 subunit beta
• Gene ID: 3688
• mRNA Refseq: NM_002211
• Protein Refseq: NP_002202
• MIM: 135630
• UniProt ID: P05556
• Chromosome Location: 10p11.2
• Pathway: Adaptive Immunity Signaling; Arf6 trafficking events; Arrhythmogenic right ventricular cardiomyopathy (ARVC); Axon guidance; CXCR4-mediated signaling events; Cell junction organization; Dilated cardiomyopathy
• Function: actin binding; alpha-actinin binding; identical protein binding; protein binding; protein domain specific binding; receptor activity

Reviews

06/24/2023

The range of applications for ITGB1 is impressive, and it can play an important role in any field.

04/19/2023

ITGB1 performed very well in Western blot, with clear bands and improved experimental accuracy.

05/29/2021

ITGB1 has a strong desensitization potential for specific allergens.

Q&As

Are there have therapeutic strategies for ITGB1 protein?

Therapeutic strategies for ITGB1 protein may include inhibiting its expression, blocking its binding to ligands, or regulating its associated signal transduction pathways. These methods may have potential applications in cancer treatment.

What are the interactions between ITGB1 protein and other proteins?

The ITGB1 protein can interact with a variety of other proteins, including ligands, proteins in signal transduction pathways, etc. These interactions enable it to regulate processes such as cell growth, differentiation, and apoptosis.

How about the specific role of ITGB1 protein in cancer?

In cancer, ITGB1 protein may play a role by promoting processes such as the proliferation, migration and invasion of tumor cells, thus promoting the occurrence and development of tumors. It could be a potential target for cancer treatment.

Which is the main function of ITGB1 protein in cells?

The main function of ITGB1 protein in cells is to act as a cell adhesion receptor, participate in the interaction between cells and extracellular matrix, and play an important role in cell migration and signal transduction.

What is the relationship between ITGB1 protein structure and function?

The structure of the ITGB1 protein determines its ability and stability to bind to ligands. The study of its structure can help us understand its function and mechanism of action.

How about the association between ITGB1 protein expression level and disease?

Recent literature has shown that the expression level of ITGB1 protein is related to the occurrence and development of a variety of diseases, including cancer, cardiovascular diseases, neurodegenerative diseases, etc.