| Cat. No. : |
TNFSF10-4752H |
| Description : |
In the field of cell biology, TNF-related apoptosis-inducing ligand (TRAIL), is a protein functioning as a ligand that induces the process of cell death called apoptosis. TRAIL has also been designated CD253 (cluster of differentiation 253). |
| Species : |
Human |
| Source : |
E.coli |
| Form : |
Lyophilized from a concentrated solution (1mg/ml) contains 150 mM NaCl, and 50mM sodium phosphate, pH 7.4. |
| Purity : |
Purity Greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
| Bio-activity : |
Recombinant Human TRAIL is fully biologically active when compared to standard. The activity is determined by the ability to induce apoptotic cell death in TRAIL-sensetive U343MG cells, ED50 for this effect is 1-3 ng/ml. |
| Physical Appearance : |
Sterile Filtered white lyophilized (freeze-dried) powder. |
| Solubility : |
Lyophilized rHuTRAIL although stable at room temperature for 3 weeks, should be stored desiccated below -18μ. Upon reconstitution rHuTRAIL should be stored at 4μ between 2-7 days and for future use below -18μ. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
| Purity Greater than 95% as determined by : |
Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
| Protein Content determined by : |
UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard. |
| Endotoxin Level : |
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug. |
| Amino acid sequence : |
MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG |
| Storage : |
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage. |
